How to make PHOSPHATE BUFFER?
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REAGENTS REQUIRED:
• Monobasic: Dissolve 2.78gm of sodium dihydrogen phosphate in 100ml of distilled water.
• Dibasic sodium phosphate (0.2M): Dissolve 5.3gm of disodium hydrogen phosphate or 7.17 gm sodium hydrogen phosphate in 100ml distilled water.
PROCEDURE:
39 ml of dihydrogen sodium phosphate is mixed with 61 ml of disodium hydrogen phosphate This made up to 200ml with distilled water .This gives phosphate (Po4)2 buffer of 0.2M. Standardized pH meter with standard buffer. Washed electrode with distilled water and introduced it into phosphate buffer prepared. The pH of the solution is 6.8.
RESULT:
Phosphate buffer was prepared and pH was observed 8.5 which was made upto 6.8 using 1N Hcl and 5N NaoH.
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REAGENTS REQUIRED:
• Monobasic: Dissolve 2.78gm of sodium dihydrogen phosphate in 100ml of distilled water.
• Dibasic sodium phosphate (0.2M): Dissolve 5.3gm of disodium hydrogen phosphate or 7.17 gm sodium hydrogen phosphate in 100ml distilled water.
PROCEDURE:
39 ml of dihydrogen sodium phosphate is mixed with 61 ml of disodium hydrogen phosphate This made up to 200ml with distilled water .This gives phosphate (Po4)2 buffer of 0.2M. Standardized pH meter with standard buffer. Washed electrode with distilled water and introduced it into phosphate buffer prepared. The pH of the solution is 6.8.
RESULT:
Phosphate buffer was prepared and pH was observed 8.5 which was made upto 6.8 using 1N Hcl and 5N NaoH.
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