What is CITRATE BUFFER? How it to be made?

    (This is practical experiment)

What is CITRATE BUFFER? How it to be made?

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REAGENT S REQUIRED: 

• Citric acid: Dissolve 2.101 gm of citric acid in 100ml distilled water.

 • Sodium citrate solution 0.1 M: Dissolved 2.941gm of sodium citrate in 100ml distilled water. 

PROCEDURE:  

46.5ml of citric acid with 3.5ml of sodium citrate solution  and upto 100ml with distilled water. It corresponds to 0.1 M citrate buffer and standardised with pH meter and measures the pH of the prepared solution. This gives citrate buffer at pH 2.5. 

RESULT: 

Citrate buffer was prepared and the pH observed was 4.8 which was adjusted to 2.5 using 1N Hcl and 5N NaoH.