(This is practical experiment)
How to prepare BARBITONE BUFFER?
REAGENTS REQUIRED:
• Diethyl barbituric acid.
• Sodium diethyl barbititrate
PROCEDURE:
Dissolve 2.85gm of diethyl barbituric acid and 14.2gm of sodium diethyl barbititrate in distilled water and upto 1 liter. This gives the barbitone buffer. The pH meter is first standararised with pH buffer. Wash electrode with distilled water and introduced into barbitone buffer prepared, the pH of solution is 6.8.
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