Insertion Vectors
Vectors that have a single target site for insertion of foreign DNA are known as insertion vectors. 20% DNA that is not required for lytic growth is removed and therefore insertion of foreign DNA resumes the size back to something like its full length and can be packaged in vitro. Maximum size of DNA that can be accommodated varies from 9- 11 kb. For DNA of larger sizes, high capacity vectors are designed like:-
Vector size. OrI Host
Cosmid. 30-45kb. Col E1 E.coli
BAC. 120-300kb. Replicon forgein E.coli
YAC 250-400kb ARS Yeast
Vectors that have a single target site for insertion of foreign DNA are known as insertion vectors. 20% DNA that is not required for lytic growth is removed and therefore insertion of foreign DNA resumes the size back to something like its full length and can be packaged in vitro. Maximum size of DNA that can be accommodated varies from 9- 11 kb. For DNA of larger sizes, high capacity vectors are designed like:-
Vector size. OrI Host
Cosmid. 30-45kb. Col E1 E.coli
BAC. 120-300kb. Replicon forgein E.coli
YAC 250-400kb ARS Yeast
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