What is Isochizomers?
Isochizomers are different Restriction endonucleases having same recognition site. In some cases, they cut identically within their recognition site, but sometime they do not. They have different optimum reaction conditions, stabilities and cost that give us an option of what to purchase. Some Restriction endonucleases recognizes only one sequence but never other, called as Ambiguous Recognition Sequence. Eg. BamH I recognize GGATCC, while Hinf I recognizes a 5bp sequence, with an eligibility of sequence starting with GA and ending with TC and having any base in between GANTC. Some REs recognition site has a site for cleavage by other Restriction Endonuclease. e.g: BamH I site GGATCC have site recognized and cleaved by Sau3A I GATC. Thus all BamH I sites can consequently be cut by Sau3A I.
No comments:
Post a Comment